TNF-alpha

• Description :

  Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) . Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

• Synonyms :

  TNF; DIF; TNFA; TNFSF2; TNF-alpha

• NCBI Gene ID :

  7124

• UniProt :

  P01375

• Accession Number :

  NP_000585.2

• Accession Number mRNA :

  NM_000594

• Chromosomal Location :

  6p21.3

• Reactivity :

  Anti-Human

• Cross Reactivity :

  Human

• Target Antigen :

  Recombinant human TNF-alpha

• Clone :

  Rabbit IgG

• Applications :

  ELISA, WB, N, IHC

• Purification Method :

  Antigen-affinity purified

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Form :

  Lyophilized from PBS

• Buffer :

  PBS

• Reconstitution :

  Water

• Storage Conditions :

  The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

• Host or Source :

  Rabbit