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TNF-alpha

CAT: 0209-300-028Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0209-300-028Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. Recombinant human Tumor Necrosis Factor-α produced in E.coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Dalton.
Synonyms
TNF; DIF; TNFA; TNFSF2; TNF-alpha
NCBI Gene ID
7124
UniProt
P01375
Accession Number
NP_000585.2
Accession Number mRNA
NM_000594
Chromosomal Location
6p21.3
Reactivity
Human
Cross Reactivity
Human
Sequence
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Assay Protocol
The lyophilized TNF-alpha is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in sterile water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at –20°C for future use.
Purity
> 95% by SDS-PAGE and RP-HPLC analysis
Bioactivity
The ED50 as determined by the proliferation assay with TF-1 cells is in the range of 0.05-0.4 ng/ml.
Length
158
Form
Lyophilized
Buffer
20 mM PB, ph 7.2 + 100 mM NaCl
Reconstitution
Water
Molecular Weight
17.5 kDa
Storage Conditions
Lyophilized Tumor Necrosis Factor-alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .
Host or Source
E. coli

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