IL-1 alpha

• Description:

  IL-1α is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant rat IL-1α is a 17.7 kDa protein containing 155 amino

• Synonyms:

  Il1a; IL-1 alpha

• NCBI Gene ID:

  24493

• UniProt:

  P16598

• Accession Number:

  NP_058715.1

• Accession Number mRNA:

  NM_017019

• Chromosomal Location:

  3q36

• Reactivity:

  Rat

• Cross Reactivity:

  Mouse, Rat

• Sequence:

  MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS

• Assay Protocol:

  Reconstitute in0.5X PBS to a concentration of 0.1-1.0mg/mL.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 as determined by the dose-dependent proliferation of murine D10S cells is < 0.005 ng/ml, corresponding to a specific activity of > 2 x 108 units/mg.

• Length:

  155

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  0.5X PBS

• Molecular Weight:

  17.7 kDa

• Host or Source:

  E. coli