Growth Hormone Receptor (hGH binding protein), soluble

• Description:

  Recombinant rbGHBP, one polypeptide chain containing 248 amino acids and having a molecular mass of ~ 28 kDa, Rabbit GHBP was purified by proprietary chromatographic techniques, (see Sakal et al. Prep Biochem Biotechnol. 30:107-23, 2000) .

• Synonyms:

  Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

• NCBI Gene ID:

  100009325

• UniProt:

  P19941

• Accession Number:

  NP_001076105.1

• Accession Number mRNA:

  NM_001082636.1

• Reactivity:

  Rabbit

• Sequence:

  AFSGSEATPATLGRASESVQRVHPGLGTNSSGKPKFTKCRSPELETFSCHWTDGVHHGLKSPGSVQLFYIRRNTQEWTQEWKECPDYVSAGENSCYFNSSYTSIWIPYCIKLTNNGGMVDQKCFSVEEIVQPDPPIGLNWTLLNVSLTGIHADIQVRWEPPPNADVQKGWIVLEYELQYKEVNETQWKMMDPVLSTSVPVYSLRLDKEYEVRVRSRQRSSEKYGEFSEVLYVTLPQMSPFTCEEDFRF

• Assay Protocol:

  It is recommended to reconstitute the lyophilized rbGHBP in sterile 0.4% NaHCO3 adjusted to pH 10, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Bioactivity:

  HGHBP is fully biologically active as evidenced by its ability of forming 2:1 complex with non-primate GHs.

• Length:

  248

• Form:

  Lyophilized

• Reconstitution:

  0.4% NaHCO3, pH 10

• Molecular Weight:

  28.0 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  APSGS