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Growth Hormone

CAT: 0209-500-022Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0209-500-022Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant zebrafish growth hormone (zbGH) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 185 amino acids with an additional Ala at its N-terminus and having a molecular mass of 21187 Dalton.
Synonyms
Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
NCBI Gene ID
407639
UniProt
Q1JQ34
Accession Number
NP_001018328.2
Accession Number mRNA
NM_001020492.2
Reactivity
Zebrafish
Sequence
AQRLFNNAVIRVQHLHQLAAKMINDFEEGLMPEERRQLSKIFPLSFCNSDSIETPTGKDETQKSSMLKLLRISFRLIESWEFPSQTLSSTISNSLTIGNPNQITEKLADLKMGISVLIKGCLDGQPNMDDNDSLPLPFEDFYLTVGETSLRESFRLLACFKKDMHKVETYLRVANCRRSLDSNCTL
Assay Protocol
It is recommended to reconstitute the lyophilized zfGH mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 99.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.
Bioactivity
ZfGH is biologically active in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.
Length
186
Form
Lyophilized
Reconstitution
Water
Molecular Weight
21.1 kDa
Host or Source
E. coli
N Terminal Sequence
AQRLFN

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