Growth Hormone

• Description:

  Recombinant zebrafish growth hormone (zbGH) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 185 amino acids with an additional Ala at its N-terminus and having a molecular mass of 21187 Dalton.

• Synonyms:

  Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

• NCBI Gene ID:

  407639

• UniProt:

  Q1JQ34

• Accession Number:

  NP_001018328.2

• Accession Number mRNA:

  NM_001020492.2

• Reactivity:

  Zebrafish

• Sequence:

  AQRLFNNAVIRVQHLHQLAAKMINDFEEGLMPEERRQLSKIFPLSFCNSDSIETPTGKDETQKSSMLKLLRISFRLIESWEFPSQTLSSTISNSLTIGNPNQITEKLADLKMGISVLIKGCLDGQPNMDDNDSLPLPFEDFYLTVGETSLRESFRLLACFKKDMHKVETYLRVANCRRSLDSNCTL

• Assay Protocol:

  It is recommended to reconstitute the lyophilized zfGH mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 99.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.

• Bioactivity:

  ZfGH is biologically active in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.

• Length:

  186

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  21.1 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  AQRLFN