Recombinant Phospho-AMPK α1, 2 (Thr183, Thr172) Monoclonal Antibody

• Background:

  The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK) . AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

• Abbreviation:

  AMPK α1, 2

• UniProt:

  Q13131; P54646

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  A synthetic peptide corresponding to residues around (Thr183, Thr172) of Human Phospho-AMPK α1, 2

• Target:

  AMPK1; PRKAA1; Tau-protein kinase PRKAA1; HMGCR kinase; ACACA kinase; AMPK subunit alpha-1; AMPKα1/2

• Clonality:

  Monoclonal, Recombinant

• Clone:

  11G1

• Conjugation:

  Unconjugated

• Applications:

  WB

• Field of Research:

  Neuroscience; Signal Transduction; Cardiovascular; Cancer; Metabolism

• Purification:

  Protein A

• Concentration:

  0.2 mg/mL

• Dilution:

  WB 1:2000-1:10000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  62 kDa,64 kDa

• Observed Molecular Weight:

  62 kDa

• Isotype:

  IgG, κ