Cdc2 (phospho Tyr15) Antibody

• Background:

  Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2.

• NCBI Gene ID:

  365842

• Swiss Prot:

  Q5XI98

• Accession Number:

  Q5XI98

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Frog

• Immunogen:

  Cdc2 (Tyr15) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Tyr15 of human, mouse, rat and xenopus cdc2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction, Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

• Calculated Molecular Weight:

  34

• Specificity:

  Cdc2 antibody is specific for cdc2 phosphorylated at Tyr15.

• Applications Notes:

  The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of SK-N-MC total cell extracts with phospho- and non-phosphopeptide competition were used to establish the phospho-specificity of the antibody. Immunolabeling of the Mr 34 kDa cdc2 protein is blocked by the Ser15 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB) . Suitability for Immunohistochemistry (IHC) has not been determined. Drosophila, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

• Symbol:

  Clk2

• NCBI Official Name:

  CDC-like kinase 2

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Sible, J.C., "Cell biology: thanks for the memory, " Nature (London), 426 (2003) 392-393.

• Background Reference 02:

  Sagata, N., "Molecular biology: untangling checkpoints, " Science, 298 (2002) 1905-1907.

• Background Reference 03:

  Nakamizo, A., Inamura, T., Inoha, S., Amano, T., Ochi, H., Ikezaki, K., and Fukui, M., "Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines, " J. Neurooncol., 59 (2002) 7-13.

• Background Reference 04:

  Maller, J.L., "Mitotic control, " Curr. Opinion. Cell Biology, 3 (1991) 269-275.

• Tested Applications:

  WB

• Protein ID:

  81910362

• Physical Properties:

  Liquid