BAFF Antibody [Buffy-1]

• NCBI Gene ID:

  10673

• Swiss Prot:

  Q9Y275

• Host:

  Rat

• Reactivity:

  Human

• Immunogen:

  BAFF (monoclonal Buffy-1) antibody was raised against recombinant human soluble BAFF (aa. 83-285) .

• Clonality:

  Monoclonal

• Clone:

  Buffy-1

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Chemokines & Cytokines, Cancer

• Purification:

  Anti BAFF (monoclonal Buffy-1) antibody was purified to ≥95% in SDS-PAGE.

• Concentration:

  Batch dependent

• Buffer:

  PBS containing 0.02% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  BAFF antibody can be stored at 4˚ C, stable for one year.

• Fragment:

  IgG2a

• Applications Notes:

  Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000) . Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.

• NCBI Official Name:

  Tumor necrosis factor (ligand) superfamily, member 13b

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999) .

• Background Reference 02:

  BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000) .

• Background Reference 03:

  A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000) .

• Tested Applications:

  WB, ICC, Flow

• Physical Properties:

  Liquid