BAFF Antibody

• Background:

  BAFF Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-κ B and c-jun N-terminal Kinase) . BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.

• NCBI Gene ID:

  10673

• Swiss Prot:

  Q9Y275

• Accession Number:

  NP_006564

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  2221

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Recombinant Protein Test (Figure 3) : Anti-BAFF antibodies (2221) detected human BAFF recombinant protein at different concentrations. Recombinant Protein Test2221 Regulated expression validation (Figure 6-8) : BAFF expression detected by anit-BAFF antibodies (2221) was up-regulated in patients with myloma or Graves’ disease or in murine transplanted heart. Regulated expression validation2221

• Field of Research:

  Chemokines & Cytokines, Cancer

• Purification:

  BAFF Antibody is Ion exchange chromatography purified.

• Positive Control:

  Cat. No. 1209 - HL60 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  BAFF Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  BAFF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human BAFF has 3 isoforms, including isoform 1 (285aa, 31kD), isoform 2 (266aa, 29kD) and isoform 3 (164aa, 17.6kD) . Mouse BAFF has 2 isoforms, including isoform 1 (309aa, 34kD) and isoform 2 (290aa, 32kD) . Rat BAFF has only one isoform (274aa, 30kD) . 2221 can detect two longer human isoforms, mouse and rat.

• Symbol:

  TNFSF13B

• Positive Control 2:

  Cat. No. 1406 - Mouse Spleen Tissue Lysate

• Positive Control 3:

  Cat. No. 17-009 - HL-60 Cell Slide

• NCBI Official Name:

  Tumor necrosis factor (ligand) superfamily, member 13b

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Moore et al. Science 1999;285:260-3.

• Background Reference 02:

  Schneider et al. J Exp Med 1999;189:1747-56.

• Background Reference 03:

  Shu et al. J Leukoc Biol 1999;65:680-3.

• Background Reference 04:

  Mukhopadhyay et al. J Biol Chem 1999 ;274:15978-81.

• Citation 01:

  16818641

• Citation 02:

  26214745

• Citation 03:

  15368291

• Citation 04:

  12867412

• Other Product Names:

  BAFF Antibody: DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator

• Tested Applications:

  ELISA, WB, ICC, IF, IHC

• Protein ID:

  5730097

• Physical Properties:

  Liquid