BAFF Antibody [Buffy-2]

• NCBI Gene ID:

  10673

• Swiss Prot:

  Q9Y275

• Host:

  Rat

• Reactivity:

  Human, Mouse

• Immunogen:

  BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285) .

• Clonality:

  Monoclonal

• Clone:

  Buffy-2

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Chemokines & Cytokines, Cancer

• Purification:

  Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.

• Concentration:

  Batch dependent

• Buffer:

  PBS containing 0.02% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  BAFF antibody can be stored at 4˚ C, stable for one year.

• Fragment:

  IgM

• Applications Notes:

  BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line) . Cells were incubated on ice for 30min in 50 μ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μ g/mL of MAb to BAFF (Buffy-2) (FITC) . After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..

• NCBI Official Name:

  Tumor necrosis factor (ligand) superfamily, member 13b

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002) .

• Background Reference 02:

  Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood 103, 689 (2004) .

• Background Reference 03:

  Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004) .

• Background Reference 04:

  BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004) .

• Tested Applications:

  WB, IHC, Flow

• Physical Properties:

  Liquid