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BAFF Antibody [Buffy-2]

CAT: 0223-XA-1012Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0223-XA-1012Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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NCBI Gene ID
10673
Swiss Prot
Q9Y275
Host
Rat
Reactivity
Human, Mouse
Immunogen
BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285) .
Clonality
Monoclonal
Clone
Buffy-2
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Chemokines & Cytokines, Cancer
Purification
Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.
Concentration
Batch dependent
Buffer
PBS containing 0.02% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
BAFF antibody can be stored at 4˚ C, stable for one year.
Fragment
IgM
Applications Notes
BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line) . Cells were incubated on ice for 30min in 50 μ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μ g/mL of MAb to BAFF (Buffy-2) (FITC) . After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..
NCBI Official Name
Tumor necrosis factor (ligand) superfamily, member 13b
NCBI Organism
Homo sapiens
Background Reference 01
Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002) .
Background Reference 02
Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood 103, 689 (2004) .
Background Reference 03
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004) .
Background Reference 04
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004) .
Tested Applications
WB, IHC, Flow
Physical Properties
Liquid

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