Phosphotyrosine Antibody [PY20] (Alkaline Phosphatase)

• Background:

  Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate.

• Host:

  Mouse

• Immunogen:

  Phosphotyrosine conjugated to carrier proteins

• Clonality:

  Monoclonal

• Clone:

  [PY20]

• Conjugation:

  Alkaline Phosphatase

• Type:

  Primary Antibodies

• Field of Research:

  Obesity, Signal Transduction, Neuroscience, Cancer

• Concentration:

  Batch dependent

• Buffer:

  Supplied in a stock solution of 50 mM Tris/1 mM MgCl2/50% glycerol, pH 8.0, containing NaN3 as a preservative

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store vial at 2-8˚ C

• Fragment:

  IgG2b

• Specificity:

  Phosphotyrosine

• Applications Notes:

  Phosphotyrosine Antibody [PY20] for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.

• Other Product Names:

  PTyr20

• Tested Applications:

  ELISA

• Physical Properties:

  Liquid