Anti-Phosphotyrosine(PY20)

Anti-Phosphotyrosine(PY20)

• Description: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: P-Tyr
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• UniProt: Not Applicable
• Host: Mouse
• Species Reactivity: Pan species
• Immunogen: Phosphotyrosine conjugated to KLH
• Target Antigen: Phosphotyrosine
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: PY20
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IF (published) | ELISA (published) | IHC, FFPE (verified) | IP (published) | WB (published)
• Validated Applications: IF, ELISA, IHC, FFPE, IP, WB
• Field of Research: Signal transduction
• Positive Control: MCF-7, MDA-231, T47-D cells or breast carcinoma
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: Depends upon the phosphorylated target
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 1-2 ug/mL|No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Immunoprecipitation 1-2 ug/500 ug protein lysate|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1983) 264(34): 20163-20166. (IP) J Immunological Meth (1988) 109: 277-285. (ELISA) Mol Endocrinol (1998) 12(4): 536-543. (WB) Fert Stert (1999) 71(5): P919-923. (IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years