C/EBP-alpha (phospho Thr230) Antibody

• Background:

  The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

• NCBI Gene ID:

  1050

• Swiss Prot:

  P49715

• Accession Number:

  NP_004355.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  C/EBP-alpha (phospho Thr230) antibody was raised against a peptide sequence around phosphorylation site of threonine 230 (P-P-T (p) -P-V) derived from Human C/EBP-alpha.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  42 kDa

• Fragment:

  IgG

• Specificity:

  The Antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at Thr230.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  CEBPA

• NCBI Official Name:

  CCAAT/enhancer binding protein (C/EBP), alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Xanthopoulos K.G. Biochem. Biophys. Res. Commun. 215:106-113 (1995)

• Background Reference 02:

  Schalwijk J. Biol. Chem. Hoppe-Seyler 378:373-379 (1997)

• Background Reference 03:

  SeattleSNPs variation discovery resource Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases

• Other Product Names:

  CCAAT/enhancer-binding protein alpha C/EBP alpha CEBPA

• Tested Applications:

  WB

• Protein ID:

  28872794

• Physical Properties:

  Liquid