C/EBP-alpha (phospho Ser21) Antibody

• Background:

  C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

• NCBI Gene ID:

  1050

• Swiss Prot:

  P49715

• Accession Number:

  NP_004355.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  C/EBP-alpha (phospho Ser21) antibody was raised against a peptide sequence around phosphorylation site of Serine 21 (L-Q-S (p) -P-P) derived from Human C/EBP-alpha.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  45 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at serine 21.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  CEBPA

• NCBI Official Name:

  CCAAT/enhancer binding protein (C/EBP), alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.

• Background Reference 02:

  Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.

• Background Reference 03:

  Raphaël Métivier, Mol. Endocrinol., Nov 2001; 15: 1953.

• Background Reference 04:

  Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.

• Other Product Names:

  CAP4, ICE8, MACH, MCH5, Mch-5

• Tested Applications:

  WB, IHC

• Protein ID:

  28872794

• Physical Properties:

  Liquid