C/EBP-ε (phospho Thr74) Antibody

• Background:

  C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

• NCBI Gene ID:

  1053

• Swiss Prot:

  Q15744

• Accession Number:

  NP_001796.2

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  C/EBP-ε (phospho Thr74) antibody was raised against a peptide sequence around phosphorylation site of threonine 174 (L-A-T (p) -A-A) derived from Human C/EBP-ε.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  34 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of C/EBP-ε only when phosphorylated at threonine 74

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  CEBPE

• NCBI Official Name:

  CCAAT/enhancer binding protein (C/EBP), epsilon

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000.

• Background Reference 02:

  Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745 - 10751.

• Background Reference 03:

  Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125 - 133.

• Background Reference 04:

  Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.

• Other Product Names:

  CEBPE, C/EBP epsilon, CCAAT/enhancer binding protein epsilon

• Tested Applications:

  WB

• Protein ID:

  28872800

• Physical Properties:

  Liquid