PKC alpha (phospho Tyr658) Antibody

• Background:

  Calcium-activated, phospholipid- and diacylglycerol (DAG) -dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells.

• NCBI Gene ID:

  5578

• Swiss Prot:

  P17252

• Accession Number:

  NP_002728.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  PKC alpha (phospho Tyr658) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 658 (F-S-Y (p) -V-N) derived from Human PKC alpha.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  80 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of PKC alpha only when phosphorylated at tyrosine 658.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  PRKCA

• NCBI Official Name:

  Protein kinase C, alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Finkenzeller G., Nucleic Acids Res. 18:2183-2183 (1990) .

• Background Reference 02:

  McSwine-Kennick R.L., J. Biol. Chem. 266:15135-15143 (1991) .

• Background Reference 03:

  Gevaert K., Nat. Biotechnol. 21:566-569 (2003)

• Other Product Names:

  PKC-alpha, KPCA, PKC III, PKC-A

• Tested Applications:

  WB

• Protein ID:

  317373571

• Physical Properties:

  Liquid