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WEE1 (phospho Ser642) Antibody

CAT: 0223-79-824Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0223-79-824Size:0.1 mg
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Background
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
NCBI Gene ID
7465
Swiss Prot
P30291
Accession Number
NP_003381.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
WEE1 (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (S-V-S (p) -L-T) derived from Human WEE1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
100 kDa
Fragment
IgG
Specificity
The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.
Applications Notes
Western Blot: 1:500~1:1000
Symbol
WEE1
NCBI Official Name
WEE1 G2 checkpoint kinase
NCBI Organism
Homo sapiens
Background Reference 01
Watanabe N., EMBO J. 14:1878-1891 (1995) .
Background Reference 02
Cichutek A., Cytogenet. Cell Genet. 93:277-283 (2001) .
Background Reference 03
Igarashi M., Nature 353:80-83 (1991)
Other Product Names
WEE1A, WEE1hu, kinase WEE1
Tested Applications
WB
Protein ID
4507917
Physical Properties
Liquid

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