Lin28 Antibody

• Background :

  Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells.

• NCBI Gene ID :

  79727

• Swiss Prot :

  Q9H9Z2

• Accession Number :

  NP_078950.1

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Lin28 antibody was raised against a peptide sequence around aa. 1~5 (M-G-S-V-S) derived from Human Lin28 .

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Stem Cell

• Purification :

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration :

  1 mg/mL

• Buffer :

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight :

  26 kDa

• Specificity :

  This antibody detects endogenous levels of total Lin28 protein.

• Applications Notes :

  Western Blot: 1:1000

• Symbol :

  LIN28A

• NCBI Official Name :

  Lin-28 homolog A (C. elegans)

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Heo I., Joo C., Mol. et al., Cell 32:276-284 (2008) Heo I., Joo C., Kim Y. -K., et al., Cell 138:696-708 (2009) Viswanathan S. R., Powers J. T., et al., Nat. Genet. 41:843-848 (2009)

• Other Product Names :

  CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A, CSDD1, LIN28A

• Tested Applications :

  WB

• Protein ID :

  13375938

• Physical Properties :

  Liquid