MEK5 Antibody

• Background:

  A family of protein kinases located upstream of the MAP kinases andresponsible for their activation has been identified. The prototype member ofthis family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present inthe Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to acti-vate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activatesboth p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

• NCBI Gene ID:

  29568

• Swiss Prot:

  Q62862

• Accession Number:

  NP_001029159.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  MEK5 antibody was raised against a peptide sequence around aa. 311-315 (K-T-Y-V-G) derived from Rat MEK5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  49 kDa

• Specificity:

  This antibody detects endogenous level of total MEK5 protein.

• Applications Notes:

  Western Blot: 1:500

• Symbol:

  Map2k5

• NCBI Official Name:

  Mitogen activated protein kinase kinase 5

• NCBI Organism:

  This antibody was raised against a peptide sequence around aa. 311-315 (K-T-Y-V-G) derived from Rat MEK5.

• Background Reference 01:

  Han, J., et al. 1996. J. Biol. Chem. 271: 2886-2891. Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926. Tournier, C., et al. 1997. Proc. Natl. Acad. Sci. USA94: 7337-7442. Holland, P. M., et al. 1997. J. Biol. Chem. 272: 24994-24998.

• Other Product Names:

  Mek5, MAP kinase kinase 5, Map2k5, MAPKK 5, MAPK/ERK kinase 5

• Tested Applications:

  WB

• Protein ID:

  77157802

• Physical Properties:

  Liquid