MEK1 / MEK2 (Ab 217 / 221) Antibody

• Background:

  Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

• NCBI Gene ID:

  5604;5605

• Swiss Prot:

  Q02750

• Accession Number:

  NP_002746.1; NP_109587.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  MEK1/MEK2 (Ab-217/221) antibody was raised against a peptide sequence around aa.215~219/219~223 (I-D-S-M-A) derived from Human MEK1/MEK2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  45 kDa

• Specificity:

  This antibody detects endogenous level of total MEK1/MEK2 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  MAP2K1 MAP2K2

• NCBI Official Name:

  Mitogen-activated protein kinase kinase 1 / mitogen-activated protein kinase kinase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shen R, et al. (2002) Mol Cell Biol; 22 (10) : 3230-3236Preisinger C, et al. (2005) EMBO J; 24 (4) : 753-765Laine P, et al. (2000) Biochem J; 349 (Pt 1) : 19-25Yaglom J, et al. (2003) Mol Cell Biol; 23 (11) : 3813-3824Dahan S, et al. (2002) Infect Immun; 70 (5) : 2304-2310

• Other Product Names:

  CFC3, MEK1, MKK1, MAPKK1, PRKMK1, GLUR2, ERK activator kinase 1/ERK activator kinase2, MAP kinase kinase 1/MAP kinase kinase 2, MAP2K1/MAP2K1, MAPK/ERK kinase 1/MAPK/ERK kinase 2, MAPKK 1/MAPKK 1

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  5579478 / 13489054

• Physical Properties:

  Liquid