MEK5 (phospho Ser311 / Thr315) Antibody

• Background:

  Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.

• NCBI Gene ID:

  29568

• Swiss Prot:

  Q62862

• Accession Number:

  NP_001029159.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  MEK5 (phospho-Ser311/ Thr315) antibody was raised against a peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S (p) I-A-K-T (p) -Y-V) derived from Rat MEK5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  46 kDa

• Specificity:

  This antibody detects endogenous level of MEK5 only when phosphorylated at serine 311 or threonine 315.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  Map2k5

• NCBI Official Name:

  Mitogen activated protein kinase kinase 5

• NCBI Organism:

  Rattus

• Background Reference 01:

  English J. M., Vanderbilt C. A., Xu S., Marcus S., Cobb M. H. J. Biol. Chem. 270:28897-28902 (1995)

• Other Product Names:

  Mek5, CDC2, CDC28A, CDKN1, P34CDC2, Map2k5

• Tested Applications:

  WB

• Protein ID:

  77157802

• Physical Properties:

  Liquid