CDK4 Antibody

CAT: 0223-79-654Size: 0.1 mgDry Ice: NoHazardous: No
CAT#:0223-79-654Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .
NCBI Gene ID
1019
Swiss Prot
P11802
Accession Number
NP_000066.1
Host
Rabbit
Reactivity
Human
Immunogen
CDK4 antibody was raised against a peptide sequence around aa.295~299 (L-H-K-D-E) derived from Human CDK4. .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
34 kDa
Specificity
This antibody detects endogenous levels of total CDK4 protein.
Applications Notes
Western Blot: 1:500~1:1000
Symbol
CDK4
NCBI Official Name
Cyclin-dependent kinase 4
NCBI Organism
Homo sapiens
Background Reference 01
Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. Sherr, C. J. (1996) Science 274, 1672-1677. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925. Gabrielli, B. G. et al. (1999) J Biol Chem 274, 13961-9.
Other Product Names
CMM3, PSK-J3, PRL3, CMM3
Tested Applications
WB
Protein ID
4502735
Physical Properties
Liquid

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