CDK7 Antibody

• Background:

  Cdk7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive carboxyl-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Cdk7 is involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

• NCBI Gene ID:

  1022

• Swiss Prot:

  P50613

• Accession Number:

  NP_001790

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDK7.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cell Cycle, Cancer, Signal Transduction

• Purification:

  Antibody is purified by Protein A/G affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CDK7 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  39 kDa

• Applications Notes:

  CDK7 antibody can be used for detection of CDK7 by ELISA at 1:75000. CDK7 antibody can be used for detection of CDK7 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  CDK7

• NCBI Official Name:

  Cyclin-dependent kinase 7

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Tassan, J.P., et al., (1994) J. Cell Biol. 127:467-478..

• Other Product Names:

  CDK7, CAK1, HCAK, MO15, STK1, CDKN7, p39MO15

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  4502743

• Physical Properties:

  Liquid