CDK9 Antibody

• Background :

  CDK9 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

• NCBI Gene ID :

  1025

• Swiss Prot :

  P50750

• Accession Number :

  NP_001252

• Host :

  Rabbit

• Reactivity :

  Human, Dog

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDK9.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cell Cycle, Cancer, Signal Transduction

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Tranfected 293T Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CDK9 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  43 kDa

• Applications Notes :

  CDK9 antibody can be used for detection of CDK9 by ELISA at 1:312500. CDK9 antibody can be used for detection of CDK9 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  CDK9

• NCBI Official Name :

  Cyclin-dependent kinase 9

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Li, Q., (2005) J. Biol. Chem. 280 (31), 28819-28826.

• Other Product Names :

  CDK9, RP11-228B15.5, C-2k, CDC2L4, PITALRE, TAK, CTK1

• Tested Applications :

  ELISA, WB

• Protein ID :

  4502747

• Physical Properties :

  Liquid