MDM2 Antibody

• Background:

  MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .

• NCBI Gene ID:

  4193

• Swiss Prot:

  Q00987

• Accession Number:

  NP_002383.2

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  MDM2 antibody was raised against a peptide sequence around aa.18~22 (Q-I-P-A-S) derived from Human MDM2. .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  90 kDa

• Specificity:

  This antibody detects endogenous levels of total MDM2 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  MDM2

• NCBI Official Name:

  MDM2 oncogene, E3 ubiquitin protein ligase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Haupt, Y. et al. (1997) Nature 387, 296-299. Mayo, L. D. and Donner, D. B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

• Other Product Names:

  HDMX, hdm2, ACTFS, CCN5, CT58, CTGFL, UNQ228/PRO261, HDMX

• Tested Applications:

  WB

• Protein ID:

  89993689

• Physical Properties:

  Liquid