ATM (phospho Ser1981) Antibody

• Background:

  ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID:

  472

• Swiss Prot:

  Q13315

• Accession Number:

  NP_000042.3

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  ATM (Phospho-Ser1981) antibody was raised against a peptide sequence around phosphorylation site of serine 1981 (E-G-S (p) -Q-S) derived from Human ATM.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  350 kDa

• Specificity:

  This antibody detects endogenous level of ATM only when phosphorylated at serine 1981.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  ATM

• NCBI Official Name:

  Ataxia telangiectasia mutated

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gupta A. et al. (2005) Mol Cell Biol. 25 (12) : 5292-5305. Bernstein JL. et al. (2002) Breast Cancer Res. 4 (6) : 249-252. Silverman J. et al. (2004) Genes Dev. 18 (17) : 2108-2119. Nakada D. et al. (2003) Nucleic Acids Res. 31 (6) : 1715-1724.

• Other Product Names:

  AT1, ATA, ATC, ATD, ATE, ATDC, TEL1, TELO1, APRF, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated, kinase ATM

• Tested Applications:

  WB, IHC

• Protein ID:

  71902540

• Physical Properties:

  Liquid