TAO2 (phospho Ser181) Antibody

• Background:

  TAO (thousand and one amino acid) protein kinases mediate signaling from carbachol to p38 MAPK and ternary complex transcription factors. This has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs. Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.

• NCBI Gene ID:

  9344

• Swiss Prot:

  Q9UL54

• Accession Number:

  Q9UL54

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  TAO2 (Ser181) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser181 of TAO2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction, Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

• Calculated Molecular Weight:

  120

• Specificity:

  TAO2 antibody is specific for Mr ~120k TAO2 phosphorylated at Ser181.

• Applications Notes:

  Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

• Symbol:

  TAOK2

• NCBI Official Name:

  TAO kinase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chen, z., Raman, M., Chen, L., Lee, S.F., Gilman, A.G., and Cobb, M.H., "TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors, " J. Biol. Chem. 278 (2003) 22,278-22,283.

• Background Reference 02:

  Chen z., and Cobb, M.H., "Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2", J. Biol. Chem. 276 (2001) 16,070-16,075.

• Background Reference 03:

  Chen, Z., Hutchison, M., and Cobb, M.H., "Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain, " J. Biol. Chem. 274 (1999) 28,803-28,807.

• Tested Applications:

  WB

• Protein ID:

  116242813

• Physical Properties:

  Liquid