NMDA (phospho Ser1480) Antibody

• Background:

  The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002) . The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993) . It has been shown that phosphorylation of Ser1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004) .

• NCBI Gene ID:

  24410

• Swiss Prot:

  Q00960

• Accession Number:

  Q00960

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish, Bovine, Chicken

• Immunogen:

  Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser1480 of the NR2B subunit of the rat NMDA receptor.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Neuroscience

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  NMDA antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  180

• Applications Notes:

  The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

• Symbol:

  Grin2b

• NCBI Official Name:

  Glutamate receptor, ionotropic, N-methyl D-aspartate 2B

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.

• Background Reference 02:

  Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.

• Background Reference 03:

  Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M, Nakanishi S (1993) Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol Chem 268:2836-2843.

• Tested Applications:

  WB

• Protein ID:

  548372

• Physical Properties:

  Liquid