FADD (phospho Ser191) Antibody

• Background:

  The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

• NCBI Gene ID:

  14082

• Swiss Prot:

  Q13158

• Accession Number:

  NP_003815.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  FADD (phospho Ser191) antibody was raised against a peptide sequence around phosphorylation site of Serine191 (R-S-G (p) -A-M) derived from Human FADD.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific , Inflammasomes

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  25 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of FADD only when phosphorylated at serine 191.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  FADD

• NCBI Official Name:

  Fas (TNFRSF6) -associated via death domain

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sugano S., Nat. Genet. 36:40-45 (2004) .

• Background Reference 02:

  Farmer A., Submitted (MAY-2003) .

• Background Reference 03:

  Venter J.C., Submitted (JUL-2005) .

• Other Product Names:

  MORT1 , FAS-associating death domain-containing protein, Mediator of receptor induced toxicity

• Tested Applications:

  WB, IHC

• Protein ID:

  6753812

• Physical Properties:

  Liquid