CPSF4 Antibody

• Background:

  Inhibition of the nuclear export of poly (A) -containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

• NCBI Gene ID:

  10898

• Swiss Prot:

  O95639

• Accession Number:

  O95639

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CPSF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-123 amino acids from the Central region of human CPSF4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Rat, Xenopus

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  30 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CPSF4

• NCBI Official Name:

  Cleavage and polyadenylation specificity factor subunit 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Twu, K.Y., et al. J. Virol. 81 (15) :8112-8121 (2007)

• Background Reference 02:

  Oh, J.H., et al. Mamm. Genome 16 (12) :942-954 (2005)

• Background Reference 03:

  Kaufmann, I., et al. EMBO J. 23 (3) :616-626 (2004)

• Background Reference 04:

  De Vries, H., et al. EMBO J. 19 (21) :5895-5904 (2000)

• Other Product Names:

  Cleavage and polyadenylation specificity factor subunit 4, Cleavage and polyadenylation specificity factor 30 kDa subunit, CPSF 30 kDa subunit, NS1 effector domain-binding protein 1, Neb-1, No arches homolog, CPSF4, CPSF30, NAR, NEB1

• Tested Applications:

  WB

• Physical Properties:

  Liquid