DIO3 Antibody

• Background:

  DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

• NCBI Gene ID:

  1735

• Swiss Prot:

  P55073

• Accession Number:

  P55073

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Neuroscience, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  34 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  DIO3

• NCBI Official Name:

  Type III iodothyronine deiodinase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bessho, K., et al. Eur. J. Pediatr. 169 (2) :215-221 (2010)

• Background Reference 02:

  Wallace, C., et al. Nat. Genet. 42 (1) :68-71 (2010)

• Background Reference 03:

  Aerts, G., et al. Endocrinology 150 (11) :5171-5180 (2009)

• Background Reference 04:

  Panicker, V., et al. J. Clin. Endocrinol. Metab. 94 (5) :1623-1629 (2009)

• Other Product Names:

  Type III iodothyronine deiodinase, 5DIII, DIOIII, Type 3 DI, Type-III 5'-deiodinase, DIO3, ITDI3, TXDI3

• Tested Applications:

  WB, IHC-P

• Protein ID:

  172045679

• Physical Properties:

  Liquid