EphB2 Antibody

• Background:

  Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.

• NCBI Gene ID:

  2048

• Swiss Prot:

  P29323

• Accession Number:

  P29323

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EphB2 antibody is generated from rabbits immunized with a recombinant human EphB2 protein.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Neuroscience, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  117 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  EPHB2

• NCBI Official Name:

  Ephrin type-B receptor 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Thanos, C.D., et al., Science 283 (5403) :833-836 (1999) .

• Background Reference 02:

  Tang, X.X., et al., Oncogene 17 (4) :521-526 (1998) .

• Background Reference 03:

  Fox, G.M., et al., Oncogene 10 (5) :897-905 (1995) .

• Background Reference 04:

  Ikegaki, N., et al., Hum. Mol. Genet. 4 (11) :2033-2045 (1995) .

• Other Product Names:

  Ephrin type-B receptor 2, Developmentally-regulated Eph-related tyrosine kinase, ELK-related tyrosine kinase, EPH tyrosine kinase 3, EPH-like kinase 5, EK5, hEK5, Renal carcinoma antigen NY-REN-47, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3, EPHB2, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5

• Tested Applications:

  WB, IHC-P, Flow

• Protein ID:

  76803654

• Physical Properties:

  Liquid