CDK4 Antibody

• Background:

  CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a) . This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb) . Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.

• NCBI Gene ID:

  1019

• Swiss Prot:

  P11802

• Accession Number:

  P11802

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-305 amino acids from the C-terminal region of human CDK4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  34 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  CDK4

• NCBI Official Name:

  Cyclin-dependent kinase 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Mori, N., et al., Int. J. Hematol. 77 (3) :259-262 (2003) .

• Background Reference 02:

  Masaki, T., et al., Hepatology 37 (3) :534-543 (2003) .

• Background Reference 03:

  Gump, J., et al., J. Biol. Chem. 278 (9) :6619-6622 (2003) .

• Background Reference 04:

  Ramirez, R.D., et al., Oncogene 22 (3) :433-444 (2003) .

• Other Product Names:

  Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4

• Tested Applications:

  WB, IHC-P, IF, Flow

• Protein ID:

  1168867

• Physical Properties:

  Liquid