CDK4 Antibody

• Background :

  CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a) . This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb) . Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.

• NCBI Gene ID :

  1019

• Swiss Prot :

  P11802

• Accession Number :

  P11802

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This CDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-305 amino acids from the C-terminal region of human CDK4.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Cell Cycle

• Purification :

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  34 kDa

• Fragment :

  Rabbit Ig

• Symbol :

  CDK4

• NCBI Official Name :

  Cyclin-dependent kinase 4

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Mori, N., et al., Int. J. Hematol. 77 (3) :259-262 (2003) .

• Background Reference 02 :

  Masaki, T., et al., Hepatology 37 (3) :534-543 (2003) .

• Background Reference 03 :

  Gump, J., et al., J. Biol. Chem. 278 (9) :6619-6622 (2003) .

• Background Reference 04 :

  Ramirez, R.D., et al., Oncogene 22 (3) :433-444 (2003) .

• Other Product Names :

  Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4

• Tested Applications :

  WB, IHC-P, IF, Flow

• Protein ID :

  1168867

• Physical Properties :

  Liquid