CDC25B Antibody

• Background:

  CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.

• NCBI Gene ID:

  994

• Swiss Prot:

  P30305

• Accession Number:

  P30305

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CDC25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from human CDC25B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  65 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CDC25B

• NCBI Official Name:

  M-phase inducer phosphatase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Uchida, S., et al., Biochem. Biophys. Res. Commun. 316 (1) :226-232 (2004) .

• Background Reference 02:

  Ito, Y., et al., Int. J. Mol. Med. 13 (3) :431-435 (2004) .

• Background Reference 03:

  Wu, W., et al., Cancer Res. 63 (19) :6195-6199 (2003) .

• Background Reference 04:

  Mils, V., et al., Exp. Cell Res. 285 (1) :99-106 (2003) .

• Other Product Names:

  M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2

• Tested Applications:

  WB

• Protein ID:

  21264471

• Physical Properties:

  Liquid