CDC45L Antibody

• Background:

  The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

• NCBI Gene ID:

  8318

• Swiss Prot:

  O75419

• Accession Number:

  O75419

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CDC45L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the Central region of human CDC45L.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  66 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  CDC45

• NCBI Official Name:

  Cell division control protein 45 homolog

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chowdhury, A., et al. Mol. Cell. Biol. 30 (6) :1495-1507 (2010)

• Background Reference 02:

  Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106 (37) :15628-15632 (2009)

• Background Reference 03:

  Ballabeni, A., et al. J. Biol. Chem. 284 (5) :3028-3036 (2009)

• Background Reference 04:

  Enjuanes, A., et al. Cancer Res. 68 (24) :10178-10186 (2008)

• Other Product Names:

  Cell division control protein 45 homolog, PORC-PI-1, CDC45, CDC45L, CDC45L2

• Tested Applications:

  WB, IHC-P, Flow

• Physical Properties:

  Liquid