SPHK1 Antibody

• Background:

  Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P) . S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

• NCBI Gene ID:

  8877

• Swiss Prot:

  Q9NYA1

• Accession Number:

  Q9NYA1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human SPHK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  43 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  SPHK1

• NCBI Official Name:

  Sphingosine kinase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ota, T., et al., Nat. Genet. 36 (1) :40-45 (2004) .

• Background Reference 02:

  Nava, V.E., et al., FEBS Lett. 473 (1) :81-84 (2000) .

• Background Reference 03:

  Melendez, A.J., et al., Gene 251 (1) :19-26 (2000) .

• Background Reference 04:

  Pitson, S.M., et al., Biochem. J. 350 Pt 2, 429-441 (2000) .

• Other Product Names:

  Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK

• Tested Applications:

  WB, IHC-P

• Protein ID:

  17369329

• Physical Properties:

  Liquid