MLLT3 Antibody

• Background:

  The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t (9;11) (p22; q23) . The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.

• NCBI Gene ID:

  4300

• Swiss Prot:

  P42568

• Accession Number:

  P42568

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MLLT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-65 amino acids from the N-terminal region of human MLLT3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  63 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MLLT3

• NCBI Official Name:

  Protein AF-9

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Iida, S., et al., Oncogene 8 (11) :3085-3092 (1993) .

• Background Reference 02:

  Nakamura, T., et al., Proc. Natl. Acad. Sci. U.S.A. 90 (10) :4631-4635 (1993) .

• Background Reference 03:

  Strissel, P. L., et al., Hum. Molec. Genet. 9: 1671-1679 (2000) .

• Other Product Names:

  Protein AF-9, ALL1-fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, YEATS domain-containing protein 3, MLLT3, AF9, YEATS3

• Tested Applications:

  WB

• Protein ID:

  215273971

• Physical Properties:

  Liquid