EPS15 Antibody

• Background:

  EPS15 is involved in cell growth regulation, possibly via the regulation of mitogenic signals and control of cell proliferation. EPS15 also participates in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Potential interaction partners include AP2A2, STN2, EPN1, and CRK via its SH3-binding sites. EPS15 is ubiquitously expressed. Phosphorylation on Tyr-849 is involved in the internalization of EGFR. EPS15 is not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. This protein is involved in a t (1;11) (p32; q23) chromosomal translocation in acute leukemias causing fusion to the trithorax (MLL or HRX) gene product which contains DNA-binding motifs resulting in a rogue activator protein. Structurally, EPS15 contains 2 EF-hand calcium-binding domains, 3 EH domains, and 2 ubiquitin-interacting motif (UIM) repeats.

• NCBI Gene ID:

  2060

• Swiss Prot:

  P42566

• Accession Number:

  P42566

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 840-870 amino acids from the C-terminal region of human EPS15.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  99 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  EPS15

• NCBI Official Name:

  Epidermal growth factor receptor substrate 15

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  De Beer, T., et al., Nat. Struct. Biol. 7 (11) :1018-1022 (2000) .

• Background Reference 02:

  Enmon, J.L., et al., Biochemistry 39 (15) :4309-4319 (2000) .

• Background Reference 03:

  De Beer, T., et al., Science 281 (5381) :1357-1360 (1998) .

• Background Reference 04:

  Chen, H., et al., Nature 394 (6695) :793-797 (1998) .

• Other Product Names:

  Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1p, EPS15, AF1P

• Tested Applications:

  WB

• Protein ID:

  67476728

• Physical Properties:

  Liquid