Granzyme B Antibody [B18.1]

• NCBI Gene ID:

  3002

• Swiss Prot:

  P10144

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Granzyme B (monoclonal B18.1) antibody was raised against recombinant human granzyme B, amino acids 7-227.

• Clonality:

  Monoclonal

• Clone:

  B18.1

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  Granzyme B (monoclonal B18.1) antibody was purified to ≥95% in SDS-PAGE.

• Concentration:

  Batch dependent

• Buffer:

  PBS containing 0.02% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Granzyme B antibody can be stored at 4˚ C, stable for one year.

• Fragment:

  IgG2a

• Applications Notes:

  Granzyme B antibody can be used for detection of human granzyme B by flow cytometry, immunocytochemistry, immunohistochemistry (frozen sections, paraffin sections), immunoprecipitation and western blot. Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 μ l FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μ g of FITC- labeled B18.1 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 μ g/mL of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Method: : Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 μ g/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

• NCBI Official Name:

  Granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation: S. Legros-Maida, et al.; Eur. J. Immunol. 24, 229 (1994) .

• Background Reference 02:

  Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients: M.V. Clement, et al.; Transplantation 57, 322 (1994) .

• Background Reference 03:

  Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor: C. Berthou, et al.; Blood 86, 3500 (1995) .

• Background Reference 04:

  Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long- term surviving lung transplanted patients: C. Berthou, et al.; Transplant. Proc. 28, 1964 (1996) .

• Tested Applications:

  WB, IHC, ICC, IP, Flow

• Physical Properties:

  Liquid