PTPN1 Antibody

• Background:

  The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.

• NCBI Gene ID:

  5770

• Swiss Prot:

  P18031

• Accession Number:

  P18031

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This PTPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-249 amino acids from the Central region of human PTPN1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  50 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PTPN1

• NCBI Official Name:

  Tyrosine-protein phosphatase non-receptor type 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Stuible, M., et al. Trends Cell Biol. 20 (11) :672-679 (2010)

• Background Reference 02:

  Jablonski, K.A., et al. Diabetes 59 (10) :2672-2681 (2010)

• Background Reference 03:

  Pradhan, S., et al. J. Biol. Chem. 285 (38) :29059-29068 (2010)

• Background Reference 04:

  Brasil, A.S., et al. Genet Test Mol Biomarkers 14 (3) :425-432 (2010)

• Other Product Names:

  Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B, PTPN1, PTP1B

• Tested Applications:

  WB

• Protein ID:

  131467

• Physical Properties:

  Liquid