PPM1F Antibody

• Background:

  The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

• NCBI Gene ID:

  9647

• Swiss Prot:

  P49593

• Accession Number:

  P49593

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PPM1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-303 amino acids from the Central region of human PPM1F.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  50 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PPM1F

• NCBI Official Name:

  Protein phosphatase 1F

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Harvey, B.P., et al. J. Biol. Chem. 279 (23) :24889-24898 (2004)

• Background Reference 02:

  Koh, C.G., et al. Curr. Biol. 12 (4) :317-321 (2002)

• Background Reference 03:

  Tan, K.M., et al. J. Biol. Chem. 276 (47) :44193-44202 (2001)

• Background Reference 04:

  Kitani, T., et al. J. Biochem. 125 (6) :1022-1028 (1999)

• Other Product Names:

  Protein phosphatase 1F, Ca (2+) /calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, PPM1F, KIAA0015, POPX2

• Tested Applications:

  WB

• Protein ID:

  24638458

• Physical Properties:

  Liquid