PPM1A Antibody

• Background:

  The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

• NCBI Gene ID:

  5494

• Swiss Prot:

  P35813

• Accession Number:

  P35813

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PPM1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-382 amino acids from human PPM1A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Rabbit

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  42 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PPM1A

• NCBI Official Name:

  Protein phosphatase 1A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 02:

  Zhang, B., et al. Histochem. Cell Biol. 132 (2) :169-179 (2009)

• Background Reference 03:

  Sun, W., et al. Cell. Signal. 21 (1) :95-102 (2009)

• Background Reference 04:

  Wang, Y., et al. J. Biol. Chem. 283 (48) :33578-33584 (2008)

• Other Product Names:

  Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, PP2C-alpha, Protein phosphatase IA, PPM1A, PPPM1A

• Tested Applications:

  WB

• Protein ID:

  548442

• Physical Properties:

  Liquid