PSMD4 Antibody

• Background:

  The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.

• NCBI Gene ID:

  5710

• Swiss Prot:

  P55036

• Accession Number:

  P55036

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PSMD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-328 amino acids from the C-terminal region of human PSMD4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  41 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PSMD4

• NCBI Official Name:

  26S proteasome non-ATPase regulatory subunit 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Elangovan, M., et al. Biochem. Biophys. Res. Commun. 396 (2) :425-428 (2010)

• Background Reference 02:

  Safadi, S.S., et al. J. Biol. Chem. 285 (2) :1424-1434 (2010)

• Background Reference 03:

  Zhang, N., et al. Mol. Cell 35 (3) :280-290 (2009)

• Background Reference 04:

  Kim, H.T., et al. EMBO J. 28 (13) :1867-1877 (2009)

• Other Product Names:

  26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, Antisecretory factor 1, AF, ASF, Multiubiquitin chain-binding protein, PSMD4, MCB1

• Tested Applications:

  WB

• Protein ID:

  1709796

• Physical Properties:

  Liquid