PSME4 Antibody

• Background:

  Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin- independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.

• NCBI Gene ID:

  23198

• Swiss Prot:

  Q14997

• Accession Number:

  Q14997

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PSME4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 503-535 amino acids from human PSME4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  211 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:2000

• Symbol:

  PSME4

• NCBI Official Name:

  Proteasome activator complex subunit 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Blickwedehl J., et al.Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.

• Background Reference 02:

  Nomura N., et al.DNA Res. 1:223-229 (1994) .

• Background Reference 03:

  Ustrell V., et al.EMBO J. 21:3516-3525 (2002) .

• Background Reference 04:

  Wang X., et al.Biochemistry 46:3553-3565 (2007) .

• Other Product Names:

  Proteasome activator complex subunit 4, Proteasome activator PA200, PSME4, KIAA0077

• Tested Applications:

  WB

• Protein ID:

  134034993

• Physical Properties:

  Liquid