CRYAA Antibody

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC) . [provided by RefSeq].

1027246521409

P02489

P02489

Human

This CRYAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human CRYAA.

Unconjugated

Primary Antibodies

Cancer, Neuroscience, Signal Transduction

This antibody is purified through a protein A column, followed by peptide affinity purification.

Batch dependent

Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rabbit, Rat

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

20 kDa

Rabbit Ig

For WB starting dilution is: 1:1000

CRYAA

Alpha-crystallin A chain

Homo sapiens

Deng, M., et al. Biochim. Biophys. Acta 1802 (7-8), 621-631 (2010) :

Li, F.F., et al. Curr. Eye Res. 35 (6) :492-498 (2010)

Pang, M., et al. Biochim. Biophys. Acta 1804 (4) :948-956 (2010)

Bhagyalaxmi, S.G., et al. OmanOphthalmol 3 (1) :7-12 (2010)

Alpha-crystallin A chain, Heat shock protein beta-4, HspB4, Alpha-crystallin A (1-172), Alpha-crystallin A (1-168), Alpha-crystallin A (1-162), CRYAA, CRYA1, HSPB4

WB

1706112