EIF3E Antibody

• Background:

  Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD) ; may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

• NCBI Gene ID:

  3646

• Swiss Prot:

  P60228

• Accession Number:

  P60228

• Host:

  Rabbit

• Reactivity:

  Human, Zebrafish

• Immunogen:

  This EIF3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the Central region of human EIF3E.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Chicken, Monkey, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  52 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  EIF3E

• NCBI Official Name:

  Eukaryotic translation initiation factor 3 subunit E {ECO:0000255|HAMAP-Rule:MF_03004}

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Grzmil, M., et al. Oncogene 29 (28) :4080-4089 (2010)

• Background Reference 02:

  Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (47) :18139-18144 (2008)

• Background Reference 03:

  Masutani, M., et al. EMBO J. 26 (14) :3373-3383 (2007)

• Background Reference 04:

  Morris, C., et al. EMBO Rep. 8 (6) :596-602 (2007)

• Other Product Names:

  Eukaryotic translation initiation factor 3 subunit E {ECO:0000255|HAMAP-Rule:MF_03004}, eIF3e {ECO:0000255|HAMAP-Rule:MF_03004}, Eukaryotic translation initiation factor 3 subunit 6 {ECO:0000255|HAMAP-Rule:MF_03004}, Viral integration site protein INT-6 homolog, eIF-3 p48 {ECO:0000255|HAMAP-Rule:MF_03004}, EIF3E {ECO:0000255|HAMAP-Rule:MF_03004}

• Tested Applications:

  WB

• Protein ID:

  41019126

• Physical Properties:

  Liquid