EIF3C Antibody

• Background:

  Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

• NCBI Gene ID:

  8663

• Swiss Prot:

  Q99613

• Accession Number:

  Q99613

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This EIF3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 538-566 amino acids from the Central region of human EIF3C.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Rat, Xenopus, Human

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  105 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  EIF3C

• NCBI Official Name:

  Eukaryotic translation initiation factor 3 subunit C {ECO:0000255|HAMAP-Rule:MF_03002}

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Imielinski, M., et al. Nat. Genet. 41 (12) :1335-1340 (2009)

• Background Reference 02:

  Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (47) :18139-18144 (2008)

• Background Reference 03:

  Masutani, M., et al. EMBO J. 26 (14) :3373-3383 (2007)

• Background Reference 04:

  Damoc, E., et al. Mol. Cell Proteomics 6 (7) :1135-1146 (2007)

• Other Product Names:

  Eukaryotic translation initiation factor 3 subunit C {ECO:0000255|HAMAP-Rule:MF_03002}, eIF3c {ECO:0000255|HAMAP-Rule:MF_03002}, Eukaryotic translation initiation factor 3 subunit 8 {ECO:0000255|HAMAP-Rule:MF_03002}, eIF3 p110 {ECO:0000255|HAMAP-Rule:MF_03002}, EIF3C {ECO:0000255|HAMAP-Rule:MF_03002}

• Tested Applications:

  WB

• Protein ID:

  6685539

• Physical Properties:

  Liquid