MBD4 Antibody

• Background:

  DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.

• NCBI Gene ID:

  8930

• Swiss Prot:

  O95243

• Accession Number:

  O95243

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MBD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the Central region of human MBD4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  66 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MBD4

• NCBI Official Name:

  Methyl-CpG-binding domain protein 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Arora, M., et al. Leukemia 24 (8) :1470-1475 (2010)

• Background Reference 02:

  Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16 (8) :1084-1089 (2010)

• Background Reference 03:

  Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :

• Background Reference 04:

  Briggs, F.B., et al. Am. J. Epidemiol. 172 (2) :217-224 (2010)

• Other Product Names:

  Methyl-CpG-binding domain protein 4, 322-, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, MBD4, MED1

• Tested Applications:

  WB

• Physical Properties:

  Liquid