MBD3 Antibody

• Background:

  DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) . The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

• NCBI Gene ID:

  53615

• Swiss Prot:

  O95983

• Accession Number:

  O95983

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-291 amino acids from the C-terminal region of human MBD3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  33 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  MBD3

• NCBI Official Name:

  Methyl-CpG-binding domain protein 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bhattacharya SK, Ramchandani S, Cervoni N, Szyf. M. Nature, 397 (6720) :579-583 1999.

• Background Reference 02:

  Hendrichand Bird A. Mol Cell Biol, 18: 6538-6547 (1998) .

• Background Reference 03:

  Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A.Biol Chem 275 (42) : 32422-32429 (2000) .

• Background Reference 04:

  Bader S, Walker M, Harrison D. BrCancer 83 (12) : 1646-1649 (2000) .

• Other Product Names:

  Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3

• Tested Applications:

  WB, IHC-P

• Physical Properties:

  Liquid