MBD3 Antibody

CAT: 0223-55-160Size: 400 µLDry Ice: NoHazardous: No
CAT#:0223-55-160Size:400 µL
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Background
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) . The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
NCBI Gene ID
53615
Swiss Prot
O95983
Accession Number
O95983
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-291 amino acids from the C-terminal region of human MBD3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
33 kDa
Fragment
Rabbit Ig
Symbol
MBD3
NCBI Official Name
Methyl-CpG-binding domain protein 3
NCBI Organism
Homo sapiens
Background Reference 01
Bhattacharya SK, Ramchandani S, Cervoni N, Szyf. M. Nature, 397 (6720) :579-583 1999.
Background Reference 02
Hendrichand Bird A. Mol Cell Biol, 18: 6538-6547 (1998) .
Background Reference 03
Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A.Biol Chem 275 (42) : 32422-32429 (2000) .
Background Reference 04
Bader S, Walker M, Harrison D. BrCancer 83 (12) : 1646-1649 (2000) .
Other Product Names
Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3
Tested Applications
WB, IHC-P
Physical Properties
Liquid

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